Components and capabilities of the ISU Confocal Facility

Leica TCS SP2 Confocal Microscope (Installed 2004)

Microscope: Inverted Leica DM IRE2 HC Fluo TCS 1-B

>> Outfitted for DIC optics and standard fluorescence
>> Galvanometer driven z-stage
>> Trinocular head and color CCD camera, allowing for capture of nonconfocal images

Objectives                                                                                                                                                                                                          

HC PL APO 20x/0.70 Multi-immersion
>> High numerical aperture Plan Apochromat Immersion lens for Water, Glycerol or Oil Immersion.

HCX PL APO 40x/1.25-0.75 Oil
>> High numerical aperture Plan Apochromat lens for Oil Immersion.

HCX PL APO 63x/1.4 - 0.6 Oil            
>> High numerical aperture Plan Apochromat lens for Oil Immersion.

HCX PL APO 63x/1.20 W Corr/-CS                   
>> High numerical aperture Plan Apochromat Immersion lens for Water Immersion with correction collar. For use without coverglass.

Confocal Scan head: TCS SP 2 Basic Module with spectral detection.

Features
3 Laser sources:
Red (HeNe 633 nm)
Light Green (561 nm DPSS)
Blue (Ar 458-476-488-514 nm)

6 channel Acousto Optical Tunable Filter (AOTF) for laser selection and attenuation.

Three channels for confocal fluorescence or reflected light.

Low-noise photomultipliers, 8 or 12 bit digitization

Transmitted light detector for recording bright field images

Spectral range of detector optics 400-800nm

Adjustable pupil illumination using Beam Expander

Variable, computer controlled detection pinhole diameter

Scan resolution up to 4096 x 4096 pixels

Zoom 1-32x

Scan rotation -2+92 degrees

Scan field diagonal 22mm (intermediate image plane)

LCS Leica Confocal Software incl. 3D, Physiology, & Multicolor packages.